50X TAE Buffer (Tris-Acetate-EDTA), 500 mL, #10417 from Cepham Life Sciences

Cepham Life Sciences 50X TAE (Tris-Acetate-EDTA) Buffer contains 2M Tris, 1M Acetic acid with 0.05M EDTA and is used for running electrophoresis of nucleic acids in the gels for providing electrical conductivity and maintaining the pH. The EDTA present in the buffer inhibits metal dependent nucleases by chelating the divalent cations (Ca2+, Mg2+), and thereby protects the DNA from nucleases during the electrophoresis. The buffer may be used for both genomic and large supercoiled DNA, and also be as a gel preparation buffer. This solution has a lower buffering capacity than TBE buffer, therefore, periodic replacement of the buffer during prolonged electrophoresis is recommended. However, dsDNA runs faster with TAE buffer than TBE. The 50X concentrated TAE buffer should be diluted with molecular biology grade water to 1X before running the electrophoresis. Available in 500 mL, 1 L, and 1 Gal sizes. For research use only. Not for human or animal clinical/therapeutic use. Please follow all safety instructions.

• Used for electrophoresis of nucleic acids in agarose & polyacrylamide gels
• Performs as both a running buffer and a gel preparation buffer
• Recommended for resolution of RNA & DNA fragments larger than 1500 bp, for genomic DNA & for large supercoiled DNA
• Ready-to-use solution provides savings in labor, raw materials, and waste disposal
• Product is guaranteed to perform as per its SOP and is backed with a money back guarantee