Glyoxalase I Assay Kit (DGLO-100)

GLYOXALASE I (GLO-1), a lactoylglutathione lyase also known as methylglyoxalase, aldoketomutase, ketone-aldehyde mutase, and (R)-S-lactoylglutathione methylglyoxal-lyase, is an enzyme that catalyzes the isomerization of hemithioacetal adducts which are formed in spontaneous reactions between glutathionyl groups and aldehydes. The primary physiological function of glyoxalase I is the detoxification of methylglyoxal, a reactive 2-oxoaldehyde that is cytostatic at low concentrations and cytotoxic at millimolar concentrations. Glyoxalase I is a target for the development of pharmaceuticals against bacteria, protozoans and human cancer. Simple, direct and automation-ready procedures for measuring GLO-1 activity in biological samples are highly desirable in research and drug discovery. BioAssay Systems' Glyoxalase I assay kit provides a sensitive and convenient method for GLO-1 activity determination. The method involves monitoring the increase in the product of the GLO-1 reaction, S-lactoylglutathione, by measuring the change in absorbance at 240 nm. This product is for Research Use only and is not intended for diagnostic purposes.

• For quantitative determination of glyoxalase activity (Use a UV plate, cat# P96UV).
• Method: Colorimetric assay (240nm). A spectrophotometer or plate reader is required, but not provided.
• Samples: Enzyme preparations or biological samples. Species: All. Detects 4 U/L
• Procedure: 30 min. Size: 100 tests per kit